1. Nguyen LV, Pellacani D, Lefort S, Kannan N, Osako T, Makarem M, Cox CL, Kennedy W, Beer P, Carles A, Moksa M, Bilenky M, Balani S, Babovic S, Sun I, Rosin M, Aparicio S, Hirst M, Eaves CJ. Barcoding reveals complex clonal dynamics of de novo transformed human mammary cells. Nature 2015. PMID: 26633636.

We were the first to demonstrate that normal human mammary epithelial cells could be transformed to invasive mammary carcinomas with just a single oncogene KRAS-G12D. We further demonstrated that there was a reversal of lineage phenotype, and that these changes on the level of individual clones could be tracked and analysed at just 2 weeks after oncogene over-expression.

3. Nguyen LV, Makarem M, Carles A, Moksa M, Kannan N, Pandoh P, Eirew P, Osako T, Kardel M, Cheung AM, Kennedy W, Tse K, Zeng T, Zhao Y, Humphries RK, Aparicio S, Eaves, CJ, Hirst M. Clonal analysis via barcoding reveals diverse growth and differentiation of transplanted mouse and human mammary stem cells. Cell Stem Cell 2014. PMID: 24440600.

We were the first to adapt lentiviral-based cellular barcoding to readout with next-generation sequencing to track hundreds of clones simultaneously. We applied this to study the heterogeneity in regenerative activity from normal mammary epithelial stem cells.